There are some key components to bluffing that often get overlooked by the micro/low stakes games.
1. Opponent types: calling stations are going to call with any hand and can never be bluffed. If you bluffed and they folded, you were almost certainly ahead anyway.
2. Your table image; if you are perceived as loose and aggressive, your opponents are more likely to look you up. You should consider how you've been playing recently, if you have been betting and raising a lot, you should seriously consider avoiding bluffing. Even if you've had the goods in your previous
hands, your opponents perception is key.
3. Board texture; does your bluff make sense? Did a scare card, like a flush or straight card come down on the river?
4. Previous streets betting; if you have check/called the flop and turn and then the flush card comes, it probably does make sense. If it's a complete brick, it almost certainly doesn't.
5. Opponents ability to read the board; this is kind of like 1., but they might be a slightly tighter player that has little or no concept of board texture and opponents range. You might be representing the flush, but if all they're paying attention to is the two pair they've got in their hand, they will call and you have to watch your chips slide across the table to them.