"Over past month I have played Nl .10 all the way up to 2.5/5 and I can say from what I have seen from myself and others that bluffing doesn’t work that often".
This subject comes up a lot, and 10NL is definitely u-stakes. 500NL not so much, but hardly a big game either, and still dominated by rec-fish. At the Micros, the players I'd target for a triple barrel stack-off are few and far between. A couple sessions ago, I pulled one off. It was early in the session at 10NL and we both had ~100BB stacks.
I opened for 3XBB from middle MP (9-handed) with red sevens, and got repopped out of the BB after the rest of the field folded. My usual inclination is to lay it down unless I figure the fish will pay off a successful set mine. I knew this player, and even though his game still has leaks, he at least is capable of thinking. I called his 3-bet. I knew more about his range than he knew about mine. Being OOP, I put his 3-bet range as:
QQ+
AK, AQ-s
Flop comes down: ( Ad, 8d, 5d ) He 2/3rds pots it. Totally standard.
Since the Ace of Trump is on board, all the suited diamonds just departed from his range. Even if he has TPTK, or even top set, I figure my flush draw is likely good even though it's seven-high. The only risks here are he flopped nuts with ( Kd,Qd ) and I'm not sure he would 3! that OOP, I figure he'd just flat instead. The only other danger is he has an accidental flush draw with ( Ax,Kd ) ( Kd,Kx ) or ( Qd,Qx ) There are more combos without the over flush draw than with it. I call.
4th St brings a black nine and I know it's a brick for his range and he doens't have any straight draws. He leads out again, and I call. Another black card brick for the river and he checks. I know that a pair of sevens isn't going to win here, so I shove into his check. This play looks like one of two things: a trapping play with a made flush. or a TPTK that was waiting to see if any more diamonds rolled off before dropping the hammer. I know more about his range than he knows about mine, and I have all the diamonds in my range, and he knows it. If he knows I know he knows that, he might have called, but he can't be sure I'm not four-leveling him, and I'm not sure he's three-leveling anyway, as he does have leaks, and not being completely observant is one of them.
It took him about five seconds to find the fold button, so I figure he had the underpairs in the pocket. He might have tanked longer with sets and TPTK.
A triple barrel bluff like this doesn't work against fish. A fish just might show you anything, even something ridiculous like ( 9d,2d ) and he 3! because boredom. A fish will definitely call with any aces, kings, or queens because aces! He'll call his pocket kings or queens because he doesn't get them that often and will figure he's gone this far, so why not? Hope springs eternal with these players. A fish thinks "range" is where the deer and antelope play, that "equity" has something to do with real estate or the stock market, and that "GTO" is a car. You don't make plays that depend on thinking opponents when those opponents are thoughtless if not downright clueless. You stick with basic, ABC poker, and take 'em to Value Town instead. Fish are unbluffable after the flop. If he's in there, he has something, and if he didn't he'd be gone. It's as simple as that.
You don't have to worry about balancing ranges or any of that fancy stuff: trust me on this: they're not paying any attention. I've seen the nittiest players sitting behind EEEEEENORMOUS stacks. He'll jam it in and some fish will call only to be shown Pocket Rockets. What else did you expect? That guy hasn't played a hand in ten orbits, and he adds another stack to his pile. I see that all the time: I'd drop Cowboys like they were on fire in that situation.
Fish don't think.
Fish notice nothing.
Just because you saw Tom "Durrrr" Dwan pull off a sick triple barrel with a river AI on Super Duper Mega Poker After Dark with the Stars doesn't mean you can do it in your 10NL game. Those are TV shows, not Poker lessons, and I can't guarantee they aren't scripted in at least part. If you open. get called, your c-bet called, and he's leading on the turn, you don't worry about defending, you don't worry you're being floated: he's got the goods, and so you fold. Don't worry that this makes you look exploitable to turn leads. They're not noticing, they're not exploiting: THEY'RE FISH.